At this point in time, most of the effort in structural biology is spent on protein expression and crystallization. Indeed, a structure usually follows quite quickly once good crystals have been obtained. Furthermore, the information content of the final structure is dependent on the quality of the crystals themselves. For this reason, the Rayment Laboratory devotes considerable time to developing the skills and enthusiasm needed to grow terrific crystals. A few of the crystals used in recent and past studies are shown here.
Myosin Subfragment 1 (PDB ID: 2MYS)
Three-dimensional structure of myosin subfragment-1: a molecular motor. Rayment, I., Rypniewski, W.R., Schmidt-Base, K., Smith, R., Tomchick, D.R., Benning, M.M., Winkelmann, D.A., Wesenberg, G., Holden, H.M. (1993) Science 261: 50-58
Transposase (PDB ID: 1MUH)
Three-dimensional structure of the Tn5 synaptic complex transposition intermediate. Davies, D.R., Goryshin, I.Y., Reznikoff, W.S., Rayment, I. (2000) Science 289: 77-85
Cnm67 (PDB ID: 3OA7)
Structure-function analysis of the C-terminal domain of CNM67, a core component of the Saccharomyces cerevisiae spindle pole body. Klenchin VA, Frye JJ, Jones MH, Winey M, Rayment I. (2011) J. Biol. Chem. 286: 18240-50.
SHD-Kar3Vik1 (PDB ID: 4ETP)
Kar3Vik1, a member of the Kinesin-14 superfamily, shows a Novel Kinesin Microtubule Binding Pattern. Rank, K.C., Chen, C.J., Cope, J., Porche, K., Hoenger, A., Gilbert, S.P., Rayment, I. (2012) J. Cell. Biol. 197: 957-970.
FtsB (PDB ID: 4IFF)
Structural organization of FtsB, a transmembrane protein of the bacterial divisome. LaPointe LM, Taylor KC, Subramaniam S, Khadria A, Rayment I, Senes A. (2013) Biochemistry 52: 2574-85.
Tropomyosin (PDB ID: 3MUD)
Structure of the tropomyosin overlap complex from chicken smooth muscle: insight into the diversity of N-terminal recognition. Frye, J., Klenchin, V.A., Rayment, I. (2010) Biochemistry 49: 4908-4920
Myosin Bound to Ulapualide (PDB ID: 1S22)
Absolute stereochemistry of ulapualide A. Allingham, J.S., Tanaka, J., Marriott, G., Rayment, I. (2004) Org. Lett. 6: 597-599
Dictyostelium Myosin II motor domain (S1dC) complexed with MgADP·BeFx (PDB ID: 1MMD)
X-ray structures of the myosin motor domain of Dictyostelium discoideum complexed with MgADP.BeFx and MgADP.AlF4-. Fisher, A.J., Smith, C.A., Thoden, J.B., Smith, R., Sutoh, K., Holden, H.M., Rayment, I. (1995) Biochemistry 34: 8960-8972